ABSTRACT
<p><b>OBJECTIVE</b>To identify the anti-inflammatory effects of artificial musk aqueous extract(AME)on lipopolysaccharide-stimulated cytokines secreted or released by RAW264.7 cells.</p><p><b>METHODS</b>Cytokines including interleukin(IL)-6,IL-10,and tumor necrosis factor Α were determined using cytokine enzyme-linked immunosorbent assay kits.</p><p><b>RESULT</b>Compared with model group,the levels of major cytokines such as tumor necrosis factor Α,IL-6,and IL-10 significantly decreased in different AME groups in a dose-dependent manner.</p><p><b>CONCLUSION</b>In lipopolysaccharide-stimulated RAW264.7 macrophages,AME can remarkably inhibit the release of inflammatory cytokines and thus exerts its anti-inflammatory effects.</p>
Subject(s)
Animals , Anti-Inflammatory Agents , Pharmacology , Cell Line, Tumor , Cytokines , Metabolism , Enzyme-Linked Immunosorbent Assay , Fatty Acids, Monounsaturated , Pharmacology , Inflammation Mediators , Metabolism , Interleukin-6 , Metabolism , Lipopolysaccharides , Macrophages , Tumor Necrosis Factor-alpha , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of Musk glucoprotein on chemotaxis of Polymorphonuclear leukocytes(PMN).</p><p><b>METHOD</b>The chemotaxis of PMN in abdominal cavity in rat induced by carboxymethyl cellulose(CMC) was used as an in vivo animal model and in in vitro it was evaluated by Boyden chamber. The concentration of cytosolic free Ca2+ was quantitated with the fluorescent Ca2+ indicator Fura-2.</p><p><b>RESULT</b>The water extract of Musk at dose of 5, 20, 80 mg.kg-1 (s.c.) significantly inhibited the chemotaxis of PMN in rat; Musk-1 at concentration of 1-100 micrograms.mL-1 can significantly inhibit the chemotaxis of rabbit PMN in vitro; Musk-1 at concentration of 1-100 micrograms.mL-1 can significantly inhibit the increase of cytosolic Ca2+ concentration in PMN of rat.</p><p><b>CONCLUSION</b>Part of mechanisms underlying antiinflammatory action of Musk is to inhibit the chemotaxis of PMN.</p>